Overexpression of tropinone reductases alters alkaloid composition in Atropa belladonna root cultures
نویسندگان
چکیده
منابع مشابه
Overexpression of tropinone reductases alters alkaloid composition in Atropa belladonna root cultures.
The medicinally applied tropane alkaloids hyoscyamine and scopolamine are produced in Atropa belladonna L. and in a small number of other Solanaceae. Calystegines are nortropane alkaloids that derive from a branching point in the tropane alkaloid biosynthetic pathway. In A. belladonna root cultures, calystegine molar concentration is 2-fold higher than that of hyoscyamine and scopolamine. In th...
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Putrescine N-methyltransferase (PMT) is the first alkaloid-specific enzyme for nicotine and tropane alkaloid formation. The pmt gene from Nicotiana tabacum was fused to the CaMV 35S promoter and integrated into the Atropa belladonna genome. Transgenic plants and derived root cultures were analysed for gene expression and for levels of alkaloids and their precursors. Scopolamine, hyoscyamine, tr...
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in journal: 1. Bálványos, I., László, I., Vida, K., Hank, H., Kursinszki, L., Tóth, E., Sz ke, É.(2001): Effect of magnesium on the genetically modified Atropa belladonna cultures. Magnesium Research 14, 309-310. IF: 0,689 2. Hank H., Bálványos I., Kursinszki L., Tóth E., Sz ke É. (2003): A táptalaj összetételének hatása transzgénikus Atropa belladonna kultúrák alkaloid produkciójára. Gyógyszer...
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Agrobacterium rhizogenes (pRi), a causative agent of hairy root disease, effectively induces hairy root formation in a variety of plant species. In our study four bacterial strains AR15834, A4, 9435 and C318 and three explants types leaf, stems and roots, were examined. Hairy roots were induced from roots, stems and leaf explants. The highest transformation efficiency of 77% was achieved by usi...
متن کاملA root-expressed L-phenylalanine:4-hydroxyphenylpyruvate aminotransferase is required for tropane alkaloid biosynthesis in Atropa belladonna.
The tropane alkaloids, hyoscyamine and scopolamine, are medicinal compounds that are the active components of several therapeutics. Hyoscyamine and scopolamine are synthesized in the roots of specific genera of the Solanaceae in a multistep pathway that is only partially elucidated. To facilitate greater understanding of tropane alkaloid biosynthesis, a de novo transcriptome assembly was develo...
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ژورنال
عنوان ژورنال: Journal of Experimental Botany
سال: 2005
ISSN: 1460-2431
DOI: 10.1093/jxb/eri067